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Inoculation and microelements: two important factors for enhanced conidiogenesis of Trichoderma asperellum in solid and liquid fermentation [Inoculación y microelementos: dos factores importantes para mejorar la conidiogénesis de Trichoderma asperellum en fermentación sólida y líquida]

dc.contributor.authorSanchez-Cuasapud D.R
dc.contributor.authorBotero-Botero L.R
dc.contributor.authorHincapié-Pérez M.
dc.date.accessioned2024-07-31T21:06:59Z
dc.date.available2024-07-31T21:06:59Z
dc.date.created2024
dc.identifier.issn3042847
dc.identifier.urihttp://hdl.handle.net/11407/8438
dc.descriptionThe standardization of cultivation processes that allow high levels of conidia growth and formation is required to formulate Trichoderma products to combat fungal diseases in agronomically important crops. This study evaluated the effects of inoculation using different inoculum concentrations (1.0x105, 1.0x106, and 1.0x107 conidia mL-1) and inoculum volumes (10, 30, and 50 mL). Later, it evaluated the effect of adding microelements (CaCO3, KH2PO4, MgSO4
dc.description7H2O, and (NH4)2SO4) on the conidiogenesis of two strains of Trichoderma asperellum (GRB-HA01 and GRB-HA02) in solid-state and liquid fermentation processes. After 12 days of fermentation, the highest conidiogenesis values for Trichoderma asperellum GRB-HA01 (6.9x109±5.7x102 conidia g-1) and Trichoderma asperellum GRB-HA02 (1.3x109±1.4x102 conidia g-1) were achieved using an inoculum volume of 10 mL at a concentration of 1.0x07 conidia mL-1. Adding CaCO3 (1 g g-1), resulted in the highest conidia concentrations for Trichoderma asperellum GRB-HA01 (3.0x1011±2.5x102 conidia g-1) and Trichoderma asperellum GRB-HA02 (8.6x1010±1.1x101 conidia g-1), reducing fermentation times to 9 days. The conidiogenesis obtained with liquid fermentation was lower and affected Trichoderma asperellum GRB-HA01 (3.1x107±1.1x102 conidia g-1) and Trichoderma asperellum GRB-HA02 (3.1x109±2.8x102 conidia g-1). This study showed that inoculation and adding microelements were important factors in the conidiogenesis processes of Trichoderma asperellum GRB-HA01 and GRB-HA02. Additionally, it was evidenced that solid-state fermentations are more efficient than liquid fermentation processes. © 2024, Universidad Nacional de Colombia. All rights reserved.
dc.language.isoeng
dc.publisherUniversidad Nacional de Colombia
dc.relation.isversionofhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85181879133&doi=10.15446%2frfnam.v77n1.108175&partnerID=40&md5=2ca9b3a09d7301882f7ee5941ba0ae89
dc.sourceRevista Facultad Nacional de Agronomia Medellin
dc.sourceRev. Fac. Nac. Agron. Medellín
dc.sourceScopus
dc.subjectCalcium carbonateeng
dc.subjectConidiaeng
dc.subjectFermentationeng
dc.subjectInoculumeng
dc.subjectPotassium dihydrogenate phosphateeng
dc.subjectTrichoderma asperellumeng
dc.titleInoculation and microelements: two important factors for enhanced conidiogenesis of Trichoderma asperellum in solid and liquid fermentation [Inoculación y microelementos: dos factores importantes para mejorar la conidiogénesis de Trichoderma asperellum en fermentación sólida y líquida]eng
dc.typearticle
dc.rights.accessrightsinfo:eu-repo/semantics/restrictedAccess
dc.publisher.programIngeniería Ambientalspa
dc.type.spaArtículo
dc.identifier.doi10.15446/rfnam.v77n1.108175
dc.relation.citationvolume77
dc.relation.citationissue1
dc.relation.citationstartpage10601
dc.relation.citationendpage10609
dc.publisher.facultyFacultad de Ingenieríasspa
dc.affiliationSanchez-Cuasapud, D.R., University of Medellin, Faculty of engineering, Research Group on Biodiversity, Biotechnology and Bioengineering GRINBIO, Medellin, Colombia
dc.affiliationBotero-Botero, L.R., University of Medellin, Faculty of engineering, Environmental Investigations and Measurements Group GEMA, Medellín, Colombia
dc.affiliationHincapié-Pérez, M.
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dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.reponamereponame:Repositorio Institucional Universidad de Medellín
dc.identifier.repourlrepourl:https://repository.udem.edu.co/
dc.identifier.instnameinstname:Universidad de Medellín


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