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Formation of 2,4-D bound residues in soils: New insights into microbial metabolism
dc.creator | Botero L.R. | spa |
dc.creator | Mougin C. | spa |
dc.creator | Peñuela G. | spa |
dc.creator | Barriuso E. | spa |
dc.date.accessioned | 2017-05-12T16:05:57Z | |
dc.date.available | 2017-05-12T16:05:57Z | |
dc.date.created | 2017 | |
dc.identifier.issn | 489697 | |
dc.identifier.uri | http://hdl.handle.net/11407/3152 | |
dc.description.abstract | The microbial contribution to the formation of bound residues in soils is studied by characterizing the metabolic activity of three microorganisms (Trametes versicolor, Fusarium solani and Ralstonia eutropha) on 14C-2,4-dichlorophenoxyacetic acid (2,4-D) during incubation in synthetic liquid media and soil. A fractionation protocol was applied to quantify the 14C-2,4-D that was incorporated into the biomass among biomolecular-like fractions. Successive fractionation of microbial biomass was implemented to break up and quantify the methanol/dichloromethane fraction (corresponding to the 14C-lipid-like fraction), the trichloroacetic acid fraction (or hydrolysed 14C-polysaccharide-like fraction) and the acid hydrolysable fraction (or the hydrolysed 14C-protein-like fraction). Relevant differences in the 2,4-D degradation and biomass radioactivity distribution among the three microorganisms were found. The 14C-protein-like fraction was the most consistent biomass fraction for reflecting the pesticide use capacity of the microorganisms under liquid and soil conditions. 2,4-D and its metabolite 4-chlorophenol were detected in methanol/dichloromethane and trichloroacetic acid fractions of the biomass of microorganisms exhibiting a low capacity to mineralize 2,4-D, thus proving that the microbial participation in the formation of bound residues while conserving the initial pesticide structure under natural soil conditions may be intimately associated with the lipid- and polysaccharide-like constituents. The fractionation protocol differentiates between 14C that is incorporated into biomass as a biomolecular constituent and the pesticide or its metabolites that accumulate in the biomass and thus correspond to the stricto sensu definition of bound residues. © 2017 Elsevier B.V. | eng |
dc.language.iso | eng | |
dc.publisher | Elsevier B.V. | spa |
dc.relation.isversionof | http://www.sciencedirect.com/science/article/pii/S0048969717301158 | spa |
dc.source | Scopus | spa |
dc.subject | 2,4-D | spa |
dc.subject | Biodegradation | spa |
dc.subject | Bound residues | spa |
dc.subject | Pesticides | spa |
dc.subject | Soil | spa |
dc.title | Formation of 2,4-D bound residues in soils: New insights into microbial metabolism | spa |
dc.type | Article in Press | eng |
dc.rights.accessrights | info:eu-repo/semantics/restrictedAccess | |
dc.rights.accessrights | info:eu-repo/semantics/restrictedAccess | |
dc.contributor.affiliation | Botero, L.R., Grupo de Investigaciones y Mediciones Ambientales, Universidad de Medellín, Carrera 87 No. 30-65, Medellín, Colombia | spa |
dc.contributor.affiliation | Mougin, C., UMR ECOSYS, INRA, AgroParisTech, Université Paris-Saclay, 78026 Versailles, France | spa |
dc.contributor.affiliation | Peñuela, G., Grupo Diagnóstico y Control de la Contaminación, Sede de Investigación Universitaria, Universidad de Antioquia, Calle 62 No. 52-59, Medellín, Colombia | spa |
dc.contributor.affiliation | Barriuso, E., UMR ECOSYS, INRA, AgroParisTech, Université Paris-Saclay, 78850 Thiverval-Grignon, France | spa |
dc.identifier.doi | 10.1016/j.scitotenv.2017.01.105 | |
dc.subject.keyword | Bacteria | eng |
dc.subject.keyword | Biodegradation | eng |
dc.subject.keyword | Biomass | eng |
dc.subject.keyword | Hydrolysis | eng |
dc.subject.keyword | Metabolism | eng |
dc.subject.keyword | Metabolites | eng |
dc.subject.keyword | Methanol | eng |
dc.subject.keyword | Microorganisms | eng |
dc.subject.keyword | Pesticides | eng |
dc.subject.keyword | Proteins | eng |
dc.subject.keyword | Soil conservation | eng |
dc.subject.keyword | Soils | eng |
dc.subject.keyword | 2 ,4-D degradation | eng |
dc.subject.keyword | 2,4-D | eng |
dc.subject.keyword | Bound residues | eng |
dc.subject.keyword | Microbial contribution | eng |
dc.subject.keyword | Microbial metabolism | eng |
dc.subject.keyword | Radioactivity distribution | eng |
dc.subject.keyword | Ralstonia eutropha | eng |
dc.subject.keyword | Trametes versicolor | eng |
dc.subject.keyword | Trichloroacetic acid | eng |
dc.relation.ispartofes | Science of the Total Environment | spa |
dc.type.driver | info:eu-repo/semantics/article |
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