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Identifying molecularly defined antigens for a Histoplasma capsulatum-specific interferon gamma release assay [Identificación de antígenos definidos molecularmente para un ensayo de liberación de interferón-gamma específico para Histoplasma capsulatum]
dc.creator | Rubio-Carrasquilla M. | |
dc.creator | Ochoa R. | |
dc.creator | Santa C. | |
dc.creator | Guimarães A.J. | |
dc.creator | Cano L.E. | |
dc.creator | Moreno E. | |
dc.date | 2019 | |
dc.date.accessioned | 2020-04-29T14:54:07Z | |
dc.date.available | 2020-04-29T14:54:07Z | |
dc.identifier.issn | 11301406 | |
dc.identifier.uri | http://hdl.handle.net/11407/5812 | |
dc.description | Background: In a previous work we showed the feasibility of an interferon gamma release assay (IGRA) for detecting latent infection by Histoplasma capsulatum. While in that proof-of-concept study we used crude fungal extracts as antigens, the newest IGRAs developed for other infections are based on molecularly defined antigens, mostly on mixtures of immunogenic peptides. Aims: To identify proteins in H. capsulatum that might serve as molecularly defined antigens for an IGRA test. Methods: We surveyed the literature looking for known H. capsulatum-immunogenic proteins and assayed two of them as antigens in an IGRA test, in a study that involved 80 volunteers. Furthermore, we used several bioinformatics tools to identify specific H. capsulatum proteins and to analyze possible strategies for the design of H. capsulatum-specific immunogenic peptides. Results: Seven H. capsulatum-immunogenic proteins were retrieved from the literature. IGRA tests using either the heat shock protein 60 or the M antigen showed high sensitivities but low specificities, most likely due to the high sequence similarity with the corresponding orthologs in other pathogenic microorganisms. We identified around 2000 H. capsulatum-specific proteins, most of which remain unannotated. Class II T-cell epitope predictions for a small number of these proteins showed a great variability among different alleles, prompting for a brute force approach for peptide design. Conclusions: The H. capsulatum genome encodes a large number of distinctive proteins, which represent a valuable source of potential specific antigens for an IGRA test. Among them, the Cfp4 protein stands out as a very attractive candidate. © 2019 Asociación Española de Micología | |
dc.language.iso | eng | |
dc.language.iso | spa | |
dc.publisher | Asociacion Espanola de Micologia | |
dc.relation.isversionof | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85075592310&doi=10.1016%2fj.riam.2019.06.002&partnerID=40&md5=af0fd57be13f4e5f724549fc8c674fa4 | |
dc.source | Revista Iberoamericana de Micologia | |
dc.subject | Histoplasma capsulatum | |
dc.subject | IGRA | |
dc.subject | Immunogenic proteins | |
dc.subject | Molecularly defined antigens | |
dc.subject | T-cell epitopes | |
dc.subject | alcohol dehydrogenase | |
dc.subject | ankyrin repeat protein | |
dc.subject | beta lactamase | |
dc.subject | chaperonin 60 | |
dc.subject | DUF636 domain containing protein | |
dc.subject | epitope | |
dc.subject | fungus antigen | |
dc.subject | M antigen | |
dc.subject | proteoglycan | |
dc.subject | synthetic peptide | |
dc.subject | unclassified drug | |
dc.subject | allele | |
dc.subject | amino acid sequence | |
dc.subject | antigen detection | |
dc.subject | antigenic variation | |
dc.subject | Article | |
dc.subject | controlled study | |
dc.subject | Histoplasma capsulatum | |
dc.subject | human | |
dc.subject | immunogenicity | |
dc.subject | interferon gamma release assay | |
dc.subject | nonhuman | |
dc.subject | orthology | |
dc.subject | pathogenicity | |
dc.subject | protein analysis | |
dc.subject | sensitivity and specificity | |
dc.subject | volunteer | |
dc.title | Identifying molecularly defined antigens for a Histoplasma capsulatum-specific interferon gamma release assay [Identificación de antígenos definidos molecularmente para un ensayo de liberación de interferón-gamma específico para Histoplasma capsulatum] | |
dc.type | Article | eng |
dc.rights.accessrights | info:eu-repo/semantics/restrictedAccess | |
dc.publisher.program | Facultad de Ciencias Básicas | |
dc.identifier.doi | 10.1016/j.riam.2019.06.002 | |
dc.relation.citationvolume | 36 | |
dc.relation.citationissue | 4 | |
dc.relation.citationstartpage | 186 | |
dc.relation.citationendpage | 191 | |
dc.publisher.faculty | Facultad de Ciencias Básicas | |
dc.affiliation | Rubio-Carrasquilla, M., Grupo de Micología Médica y Experimental, Corporación para Investigaciones Biológicas (CIB), Medellín, Colombia, Instituto de Biología, Universidad de Antioquia, Medellín, Colombia; Ochoa, R., Programa de Estudio y Control de Enfermedades Tropicales PECET, Universidad de Antioquia, Medellín, Colombia; Santa, C., Universidad Nacional de Colombia, Sede Medellín, Medellín, Colombia; Guimarães, A.J., Depto de Microbiologia e Parasitologia, Universidade Federal Fluminense, Niterói, RJ, Brazil; Cano, L.E., Grupo de Micología Médica y Experimental, Corporación para Investigaciones Biológicas (CIB), Medellín, Colombia, Escuela de Microbiología, Universidad de Antioquia, Medellín, Colombia; Moreno, E., Facultad de Ciencias Básicas, Universidad de Medellín, Medellín, Colombia | |
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dc.type.version | info:eu-repo/semantics/publishedVersion | |
dc.type.driver | info:eu-repo/semantics/article |
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