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Structural Insights into the Design of Synthetic Nanobody Libraries

dc.contributor.authorValdés-Tresanco M.S
dc.contributor.authorMolina-Zapata A
dc.contributor.authorPose A.G
dc.contributor.authorMoreno E.
dc.date.accessioned2022-09-14T14:34:09Z
dc.date.available2022-09-14T14:34:09Z
dc.date.created2022
dc.identifier.issn14203049
dc.identifier.urihttp://hdl.handle.net/11407/7583
dc.descriptionSingle domain antibodies from camelids, or nanobodies, are a unique class of antibody fragments with several advantageous characteristics: small monomeric size, high stability and solubility and easy tailoring for multiple applications. Nanobodies are gaining increasing acceptance as diagnostic tools and promising therapeutic agents in cancer and other diseases. While most nanobodies are obtained from immunized animals of the camelid family, a few synthetic nanobody libraries constructed in recent years have shown the capability of generating high quality nanobodies in terms of affinity and stability. Since this synthetic approach has important advantages over the use of animals, the recent advances are indeed encouraging. Here we review over a dozen synthetic nanobody libraries reported so far and discuss the different approaches followed in their construction and validation, with an emphasis on framework and hypervariable loop design as critical issues defining their potential as high-class nanobody sources. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.eng
dc.language.isoeng
dc.publisherMDPI
dc.relation.isversionofhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85127879154&doi=10.3390%2fmolecules27072198&partnerID=40&md5=500fd71e9664896d28605a8d04237e7b
dc.sourceMolecules
dc.titleStructural Insights into the Design of Synthetic Nanobody Libraries
dc.typeReview
dc.rights.accessrightsinfo:eu-repo/semantics/restrictedAccess
dc.publisher.programCiencias Básicas
dc.type.spaRevisión
dc.identifier.doi10.3390/molecules27072198
dc.subject.keywordNanobodyeng
dc.subject.keywordPhage displayeng
dc.subject.keywordRational designeng
dc.subject.keywordRibosome displayeng
dc.subject.keywordSynthetic libraryeng
dc.relation.citationvolume27
dc.relation.citationissue7
dc.publisher.facultyFacultad de Ciencias Básicas
dc.affiliationValdés-Tresanco, M.S., Faculty of Basic Sciences, University of Medellin, Medellin, 050026, Colombia
dc.affiliationMolina-Zapata, A., Faculty of Basic Sciences, University of Medellin, Medellin, 050026, Colombia, Grupo de Micología Médica y Experimental, Corporación para Investigaciones Biológicas (CIB), Medellin, 050034, Colombia
dc.affiliationPose, A.G., Faculty of Basic Sciences, University of Medellin, Medellin, 050026, Colombia
dc.affiliationMoreno, E., Faculty of Basic Sciences, University of Medellin, Medellin, 050026, Colombia
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