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New Formulation of a Subunit Vaccine Candidate against Lawsonia intracellularis Increases Humoral and Cellular Immune Responses

dc.contributor.authorSalazar S
dc.contributor.authorStarck M.F
dc.contributor.authorVillegas M.F
dc.contributor.authorAcosta J
dc.contributor.authorSánchez O
dc.contributor.authorRamos E
dc.contributor.authorNova-Lamperti E
dc.contributor.authorToledo J.R
dc.contributor.authorGädicke P
dc.contributor.authorRuiz Á
dc.contributor.authorGonzález A
dc.contributor.authorMontesino R.
dc.date.accessioned2024-07-31T21:07:20Z
dc.date.available2024-07-31T21:07:20Z
dc.date.created2023
dc.identifier.issn2076393X
dc.identifier.urihttp://hdl.handle.net/11407/8545
dc.descriptionPreviously, we designed a subunit vaccine candidate based on three L. intracellularis antigens with promising results in pigs. In this study, antigens were produced individually to achieve an even antigen ratio in the formulation. The emulsion characterization included the drop size and the mechanical and thermal stability. Immune response was evaluated by indirect and sandwich ELISAs, qPCR, and flow cytometry. The vaccine candidate’s safety was assessed by histopathology and monitoring the clinical behavior of animals. The average production yielded for the chimeric antigen as inclusion bodies was around 75 mg/L. The formulation showed mechanical and thermal stability, with a ratio Hu/Ho > 0.85 and a drop size under 0.15 nm. Antigens formulated at a ratio of 1:1:1 induced a significant immune response in inoculated pigs that persisted until the end of the experiment (week 14). The dose of 200 μg significantly activated cellular response measured by transcriptional and translational levels of cytokines. The cell proliferation assay revealed an increment of lymphocytes T CD4+ at the same dose. Animals gained weight constantly and showed proper clinical behavior during immunization assays. This research demonstrated the immunological robustness of the new subunit vaccine candidate against Porcine Proliferative Enteropathy evenly formulated with three chimeric antigens of L. intracellularis. © 2023 by the authors.
dc.language.isoeng
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI)
dc.relation.isversionofhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85180710391&doi=10.3390%2fvaccines11121817&partnerID=40&md5=3582a02ec0896bb97efdaa209384f1eb
dc.sourceVaccines
dc.sourceVaccines
dc.sourceScopus
dc.subjectISA 660 VGeng
dc.subjectLawsonia intracellulariseng
dc.subjectPig immune responseeng
dc.subjectUbunit vaccineeng
dc.subjectWater-in-oil formulationeng
dc.subjectCytokineeng
dc.subjectDNA 16Seng
dc.subjectEpitopeeng
dc.subjectGamma interferoneng
dc.subjectGentamicineng
dc.subjectInterleukin 12eng
dc.subjectInterleukin 4eng
dc.subjectMajor histocompatibility antigen class 1eng
dc.subjectRecombinant proteineng
dc.subjectSubunit vaccineeng
dc.subjectThiomersaleng
dc.subjectAnimal celleng
dc.subjectAnimal experimenteng
dc.subjectAnimal modeleng
dc.subjectAntibiotic resistanceeng
dc.subjectAntibody responseeng
dc.subjectAntibody titereng
dc.subjectArticleeng
dc.subjectBacterial genomeeng
dc.subjectBacterium contaminationeng
dc.subjectCell inclusioneng
dc.subjectCell proliferationeng
dc.subjectCell proliferation assayeng
dc.subjectCellular immunityeng
dc.subjectChimeraeng
dc.subjectControlled studyeng
dc.subjectDNA replication origineng
dc.subjectDrug formulationeng
dc.subjectEmulsioneng
dc.subjectEscherichia colieng
dc.subjectExpression vectoreng
dc.subjectFemaleeng
dc.subjectFlow cytometryeng
dc.subjectGene expressioneng
dc.subjectHistopathologyeng
dc.subjectHumoral immunityeng
dc.subjectHybridomaeng
dc.subjectImmune responseeng
dc.subjectImmunizationeng
dc.subjectImmunogenicityeng
dc.subjectInoculationeng
dc.subjectIntestine floraeng
dc.subjectLawsonia intracellulariseng
dc.subjectMouseeng
dc.subjectNonhumaneng
dc.subjectParasitismeng
dc.subjectPeripheral blood mononuclear celleng
dc.subjectPorcine epidemic diarrhea viruseng
dc.subjectProtein purificationeng
dc.subjectReal time polymerase chain reactioneng
dc.subjectSandwich ELISAeng
dc.subjectSeroconversioneng
dc.subjectSpleen celleng
dc.subjectThermostabilityeng
dc.subjectVaccinationeng
dc.subjectVaccine developmenteng
dc.subjectVirus sheddingeng
dc.titleNew Formulation of a Subunit Vaccine Candidate against Lawsonia intracellularis Increases Humoral and Cellular Immune Responseseng
dc.typearticle
dc.rights.accessrightsinfo:eu-repo/semantics/restrictedAccess
dc.type.spaArtículo
dc.identifier.doi10.3390/vaccines11121817
dc.relation.citationvolume11
dc.relation.citationissue12
dc.publisher.facultyFacultad de Ciencias Básicasspa
dc.affiliationSalazar, S., Biotechnology and Biopharmaceuticals Laboratory, Pathophysiology Department, School of Biological Sciences, Universidad de Concepción, Victor Lamas 1290, Concepción P.O. Box 160-C, Chile
dc.affiliationStarck, M.F., Biotechnology and Biopharmaceuticals Laboratory, Pathophysiology Department, School of Biological Sciences, Universidad de Concepción, Victor Lamas 1290, Concepción P.O. Box 160-C, Chile
dc.affiliationVillegas, M.F., Biotechnology and Biopharmaceuticals Laboratory, Pathophysiology Department, School of Biological Sciences, Universidad de Concepción, Victor Lamas 1290, Concepción P.O. Box 160-C, Chile
dc.affiliationAcosta, J., Biotechnology and Biopharmaceuticals Laboratory, Pathophysiology Department, School of Biological Sciences, Universidad de Concepción, Victor Lamas 1290, Concepción P.O. Box 160-C, Chile
dc.affiliationSánchez, O., Pharmacology Department, School of Biological Sciences, Universidad de Concepción, Victor Lamas 1290, Concepción P.O. Box 160-C, Chile
dc.affiliationRamos, E., Biotechnology and Biopharmaceuticals Laboratory, Pathophysiology Department, School of Biological Sciences, Universidad de Concepción, Victor Lamas 1290, Concepción P.O. Box 160-C, Chile
dc.affiliationNova-Lamperti, E., Molecular and Translational Immunology Laboratory, Clinical Biochemistry and Immunology Department, Pharmacy Faculty, Universidad de Concepción, Victor Lamas 1290,, P.O. Box 160-C, Concepción, Chile
dc.affiliationToledo, J.R., Biotechnology and Biopharmaceuticals Laboratory, Pathophysiology Department, School of Biological Sciences, Universidad de Concepción, Victor Lamas 1290, Concepción P.O. Box 160-C, Chile
dc.affiliationGädicke, P., Pathology and Preventive Medicine Department, School of Veterinary Sciences, Universidad de Concepción, Avenida Vicente Méndez 595, Chillan P.O. Box 537, Chile
dc.affiliationRuiz, Á., Pathology and Preventive Medicine Department, School of Veterinary Sciences, Universidad de Concepción, Avenida Vicente Méndez 595, Chillan P.O. Box 537, Chile
dc.affiliationGonzález, A., Faculty of Basic Sciences, University of Medellin, Cra. 87 No. 30-65, Medellin P.C. 050026, Antioquia, Colombia
dc.affiliationMontesino, R., Biotechnology and Biopharmaceuticals Laboratory, Pathophysiology Department, School of Biological Sciences, Universidad de Concepción, Victor Lamas 1290, Concepción P.O. Box 160-C, Chile
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dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.reponamereponame:Repositorio Institucional Universidad de Medellín
dc.identifier.repourlrepourl:https://repository.udem.edu.co/
dc.identifier.instnameinstname:Universidad de Medellín


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