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Selección de nanoanticuerpos contra antígenos tumorales mediante la utilización de una biblioteca sintética expresada en la superficie de fagos filamentosos

dc.contributor.advisorGonzález Pose, Alain Antonio
dc.contributor.advisorMoreno Frías, Ernesto
dc.contributor.advisorCamacho Casanova, Frank
dc.contributor.authorSalazar Uribe, Julieta
dc.coverage.spatialLat: 06 15 00 N degrees minutes Lat: 6.2500 decimal degrees Long: 075 36 00 W degrees minutes Long: -75.6000 decimal degreeseng
dc.coverage.spatialLat: 06 15 00 N  degrees minutes  Lat: 6.2500  decimal degreesLong: 075 36 00 W  degrees minutes  Long: -75.6000  decimal degrees
dc.date2023-06-14
dc.date.accessioned2024-03-04T13:53:39Z
dc.date.available2024-03-04T13:53:39Z
dc.identifier.otherT 0438 2023
dc.identifier.urihttp://hdl.handle.net/11407/8296
dc.descriptionEl factor de crecimiento epidérmico (EGF) se produce principalmente de forma paracrina y su concentración en suero humano es muy variable, pero tiende a ser mayor en paciente con cáncer. Es uno de los ligandos más importantes del receptor del factor de crecimiento epidérmico (EGFR). Otro antígeno importante en el desarrollo de cáncer es el factor de necrosis tumoral alfa TNFα como una citocina proinflamatoria. El TNFα es una citocina de 17 kDa con 157 aminoácidos, producido principalmente por macrófagos activados, linfocitos T y células asesinas naturales (NK), también en menor cantidad por células tumorales. Con el objetivo de generar nanoanticuerpos específicos contra diversos antígenos a partir de una biblioteca sintética mediante la tecnología de la presentación de proteínas en la superficie de fagos filamentosos, se realizaron dos estrategias de selección. Las variaciones entre las estrategias utilizadas consistieron en modificaciones en los lavados, las eluciones y la cantidad de rondas de enriquecimiento Utilizando cuatro eluciones diferentes consecutivas y tres rondas de enriquecimiento obtuvimos 4 nanoanticuerpos específicos de EGF, mientras que utilizando una única ronda de selección y condiciones astringentes de lavado y elución logramos obtener 21 nanoanticuerpos específicos de TNFα. La metodología de elución que se emplea durante la realización de la técnica de Phage Display podría influir en la diversidad de secuencias de los fagos que reconocen el antígeno de interés.spa
dc.format.extentp. 1-113
dc.format.mediumElectrónico
dc.format.mimetypeapplication/pdf
dc.language.isospa
dc.publisherUniversidad de Medellínspa
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0*
dc.subjectNanoanticuerposspa
dc.subjectBiblioteca sintéticaspa
dc.subjectEnriquecimiento por afinidadspa
dc.subjectPhage displayspa
dc.subjectBacteriófagospa
dc.subjectAntígenos tumoralesspa
dc.subjectFactor de crecimiento epidérmico EGFspa
dc.titleSelección de nanoanticuerpos contra antígenos tumorales mediante la utilización de una biblioteca sintética expresada en la superficie de fagos filamentososspa
dc.rights.accessrightsinfo:eurepo/semantics/openAccess
dc.publisher.programMaestría en Modelación y Ciencia Computacionalspa
dc.subject.lembAnticuerposspa
dc.subject.lembAnticuerpos monoclonalesspa
dc.subject.lembAntígenos tumoralesspa
dc.subject.lembBacteriófagosspa
dc.subject.lembBiblioteca de péptidosspa
dc.subject.lembCáncer - Aspectos genéticosspa
dc.subject.lembClones (Biología)spa
dc.relation.citationstartpage1
dc.relation.citationendpage113
dc.audienceComunidad Universidad de Medellínspa
dc.publisher.facultyFacultad de Ciencias Básicasspa
dc.publisher.placeMedellínspa
dc.type.hasversionpublishedVersion
dc.type.hasversioninfo:eu-repo/semantics/acceptedVersion
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dc.rights.creativecommonsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.type.coarhttp://purl.org/coar/resource_type/c_bdcc
dc.type.localTesis de Maestría
dc.type.driverinfo:eu-repo/semantics/masterThesis
dc.identifier.reponamereponame:Repositorio Institucional Universidad de Medellínspa
dc.identifier.instnameinstname:Universidad de Medellínspa
dc.description.degreenameMagíster en Modelación y Ciencia Computacionalspa
dc.description.degreelevelMaestríaspa
dc.publisher.grantorUniversidad de Medellínspa


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